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Proteomic analysis of multiple sclerosis cerebrospinal fluid

Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA, barbara.hammack{at}ushsc.edu

K YC Fung

Biochemical Mass Spectrometry Facility, University of Colorado Health Sciences Center, Denver, CO 80262, USA

S W Hunsucker

Biochemical Mass Spectrometry Facility, University of Colorado Health Sciences Center, Denver, CO 80262, USA

M W Duncan

Biochemical Mass Spectrometry Facility, University of Colorado Health Sciences Center, Denver, CO 80262, USA

M P Burgoon

Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA

G P Owens

Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA

D H Gilden

Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA, Department of Microbiology, University of Colorado Health Sciences Center, Denver, CO 80262, USA

Two-dimensional gel electrophoresis and peptide mass fingerprinting were used to identify proteins in cerebrospinal fluid (C SF) pooled from three patients with multiple sclerosis (MS) and in C SF pooled from three patients with non-MS inflammatory central nervous system (C NS) disorders. Resolution of C SF proteins on three pH gradients (3-10, 4-7 and 6-11) enabled identification of a total of 430 spots in the MS C SF proteome that represented 61 distinct proteins. The gels containing MS C SF revealed 103 protein spots that were not seen on control gels. A ll but four of these 103 spots were proteins known to be present in normal human C SF. The four exceptio ns were: C RTAC -1B (cartilage acidic protein), tetranectin (a plasminogen-binding protein), SPARC -like protein (a calcium binding cell signalling glycoprotein), and autotaxin t (a phosphodiesterase). It remains unknown whether these four proteins are related to the cause and patho genesis of MS.

Key Words: cerebrospinal fluid • multiple sclerosis • proteomics

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