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Increase of CD4+TNF+IL-2–T cells in cerebrospinal fluid of multiple sclerosis patients

Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

Y Kawano

Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

T Matsuoka

Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

FJ Mei

Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

T Ishizu

Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

Y Ohyagi

Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

J Kira

Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan kira{at}neuro.med.kyushu-u.ac.jp

Intracellular production of TNF and IL-2 after stimulation with phorbol myristate/ionomycin was flowcytometrically measured in CD4⁺ T cells from peripheral blood (PB) and cerebrospinal fluid (CSF) of 29 patients with multiple sclerosis (MS), and 16 with other inflammatory and 41 with other non-inflammatory neurological diseases. In CSF, the percentages of CD4⁺TNF⁺IL-2^–T cells were significantly higher in patients with MS than either of the controls, whereas no difference was found in CD4⁺TNF⁺IL-2⁺T or CD4⁺TNF^–IL-2⁺T cells. The increase was more pronounced at relapse than in remission. No significant change was detected in PB. These findings suggested that CD4⁺TNF⁺IL-2^–T cells are intrathecally upregulated in MS.

Key Words: cerebrospinal fluid • flow cytometry • IL-2 • multiple sclerosis • TNF

This version was published on January 1, 2009

Multiple Sclerosis, Vol. 15, No. 1, 120-123 (2009)
DOI: 10.1177/1352458508096871


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