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Experimental autoimmune encephalomyelitis-induced upregulation of tumor necrosis factor-alpha in the dorsal root gangliaFaculty of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, MB, Canada, Department of Neurology Multiple Sclerosis Clinic, Health Sciences Centre, University of Manitoba, Winnipeg, MB, Canada
Faculty of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, MB, Canada
Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada, Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, MB, Canada, Manitoba Institute of Child Health, University of Manitoba, Winnipeg, MB, Canada
Faculty of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, MB, Canada
Faculty of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, MB, Canada
Faculty of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, MB, Canada, Manitoba Institute of Child Health, University of Manitoba, Winnipeg, MB, Canada
Faculty of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, MB, Canada, Manitoba Institute of Child Health, University of Manitoba, Winnipeg, MB, Canada
Manitoba Institute of Child Health, University of Manitoba, Winnipeg, MB, Canada, Department of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada
Department of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada, froste{at}cc.umanitoba.ca, Department of Pathology, University of Manitoba, Winnipeg, MB, Canada
Faculty of Pharmacy, Apotex Centre, University of Manitoba, Winnipeg, MB, Canada, Department of Neurology Multiple Sclerosis Clinic, Health Sciences Centre, University of Manitoba, Winnipeg, MB, Canada, Manitoba Institute of Child Health, University of Manitoba, Winnipeg, MB, Canada Background: Multiple sclerosis (MS) is a chronic, neurological disease characterized by targeted destruction of central nervous system (CNS) myelin. The autoimmune theory is the most widely accepted explanation of disease pathology. Circulating Th1 cells become activated by exposure to CNS-specific antigens such as myelin basic protein. The activated Th1 cells secrete inflammatory cytokines, which are pivotal for inflammatory responses. We hypothesize that enhanced production of inflammatory cytokines triggers cellular events within the dorsal root ganglia (DRG) and/or spinal cord, facilitating the development of neuropathic pain (NPP) in MS. NPP, the second worst disease-induced symptom suffered by patients with MS, is normally regulated by DRG and/or spinal cord.
Objective: To determine gene and protein expression levels of tumor necrosis factor-alpha (TNF Methods: Experimental autoimmune encephalomyelitis (EAE) was induced in adolescent female Lewis rats. Animals were sacrificed every 3 days post-disease induction. DRG and spinal cords were harvested for protein and gene expression analysis.
Results: We show significant increases in TNF
Conclusion: Antigen-induced production of inflammatory cytokines such as TNF
Key Words: DRG • EAE • multiple sclerosis • neuropathic pain • tumor necrosis factor-alpha
This version was published on October
1, 2009 Multiple Sclerosis, Vol. 15, No. 10,
1135-1145 (2009) |
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) within DRG and/or spinal cord in an animal model of MS.