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Oligoclonal expansion of T cells in cerebrospinal fluid of multiple sclerosis patients

The Maria Skiodowska-Curie Memorial Cancer Center and Institute of Oncology, Wawelska 15, 00–973; Institute of Psychiatry and Neurology, 2nd Department of Neurology, Sobieskiego 1/9, Warsaw, Poland

Katarzyna Bieganowska

The Maria Skiodowska-Curie Memorial Cancer Center and Institute of Oncology, Wawelska 15, 00–973; Institute of Psychiatry and Neurology, 2nd Department of Neurology, Sobieskiego 1/9, Warsaw, Poland

Jacek Zaborski

The Maria Skiodowska-Curie Memorial Cancer Center and Institute of Oncology, Wawelska 15, 00–973; Institute of Psychiatry and Neurology, 2nd Department of Neurology, Sobieskiego 1/9, Warsaw, Poland

Anna Czionkowska

The Maria Skiodowska-Curie Memorial Cancer Center and Institute of Oncology, Wawelska 15, 00–973; Institute of Psychiatry and Neurology, 2nd Department of Neurology, Sobieskiego 1/9, Warsaw, Poland

We have used a PCR based method to analyse TCR chain repertoire and clonality of T cells in the CSF and blood of 11 MS patients. Samples collected from nine patients with other neurological diseases were used as a control. Five controls had central nervous system inflammation and four had non-inflammatory processes. We have observed a decreased percentage of T cells expressing TCR with V₉ and J_p fragments in the CSF samples in comparison with the blood. We did not find clonal expansion of the cells in any control case. Clonal expansion of T cells occurred in five of 11 MS cases in the CSF but not in the blood. Two of these clones expressed TCR rearranged with V₉ and J₁ fragments, two others used V₁₀ and J_p1, and one used V₉ and J_p fragments. We found no correlation between clonality and clinical state of patients, duration of the disease or number of cells in CSF. Our study provides additional evidence for the possible role of the T cells in the MS pathogenesis.

Key Words: multiple sclerosis • T cell • cerebrospinal fluid • PCR

Multiple Sclerosis, Vol. 2, No. 2, 78-82 (1996)
DOI: 10.1177/135245859600200203


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