This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Vora, A. Articles by Brown, K. Search for Related Content PubMed PubMed Citation Articles by Vora, A. Articles by Brown, K. Social Bookmarking                         What's this?

Lymphocyte-endothelial cell interactions in multiple sclerosis: disease specificity and relationship to circulating tumour necrosis factor- and soluble adhesion molecules

Department of Immunology, The Rayne Institute, UMDS, St Thomas' Hospital Campus, London SE1 7EH

D. Kidd

Institute of Neurology, Queen Square, London WC1N 3BG

DH Miller

Institute of Neurology, Queen Square, London WC1N 3BG

GD Perkin

Department of Neurology, Charing Cross Hospital, London W6 8RF

Rac Hughes

Department of Neurology, UMDS, Guy's and St Thomas' Hospital Trust, London SE1 9RT, UK

BA Ellis

Department of Immunology, The Rayne Institute, UMDS, St Thomas' Hospital Campus, London SE1 7EH

DC Dumonde

Department of Immunology, The Rayne Institute, UMDS, St Thomas' Hospital Campus, London SE1 7EH

KA Brown

Department of Immunology, The Rayne Institute, UMDS, St Thomas' Hospital Campus, London SE1 7EH

This study addressed two questions: first, whether the supranormal adherence of blood lymphocytes from patients with multiple sderosis (MS) to endothelial cell monolayers treated with tumour necrosis factor- (TNF) was a feature common to other inflammatory disorders; and second, whether the adherence properties of blood lymphocytes from MS patients were related to changes in disease activity and to levels of circulating TNF and soluble adhesion molecules. In the first part of the investigation, lymphocytes from I 4 patients with MS were more adherent to TNF-treated endothelial cells (P < 0.01) than those from healthy controls, whereas the adherence properties of lymphocytes from 12 patients with rheumatoid arthritis, eight patients with psoriasis and ten patients with neurological diseases other than MS were normal. In the second phase of the work, measurement of the adhesive properties of lymphocytes isolated at monthly intervals from a further six MS patients over a 5 - 8 month period, found that changes in binding to TNF-treated endothelial cells, directly paralleled changes in circulating levels of TNF (r =0.77; P < 0.001) and soluble vascular cell adhesion molecule-I (sVCAM-I) (r=0.67; P=0.001). An increase in disease activity, measured by T2-weighted and gadolinium-enhanced magnetic resonance imaging of the central nervous system (CNS), occurred in two patients and was associated with heightened lymphocyte adhesiveness and a rise in serum TNF levels. Further analysis of the 34 serum samples from the six MS patients revealed a direct relationship between the concentration of sL-selectin and soluble intercellular adhesion molecule- (sICAM-I) (r=0.65; P < 0.001) and between sL-selectin and sTNF (r=0.42; P < 0.02). These findings support the view that disease activity in MS is associated with an increased adhesive interaction of blood lymphocytes with vascular endothelium at inflammatory sites within the CNS.

Key Words: adherence • disease activity • endothelial cells • lymphocytes • multiple sclerosis • tumour necrosis factor

Multiple Sclerosis, Vol. 3, No. 3, 171-179 (1997)
DOI: 10.1177/135245859700300301


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?