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Identification of peptides binding to IgG in the CSF of Multiple Sclerosis patients

Clinica Neurologica, Università di Roma TorVergata, 00100 Roma, and Ospedale di Riabilitazione S. Lucia, via Ardeatina 306, 00173 Roma, Italy

S Capone

Istituto di Ricerche di Biologia Molecolare P.Angeletti, Via Pontina Km. 30.6, 00040 Pomezia RM, Italy

R Tafi

Istituto di Ricerche di Biologia Molecolare P.Angeletti, Via Pontina Km. 30.6, 00040 Pomezia RM, Italy

L ME Grimaldi

Neuroimmunology Unit - DIBIT, Department of Neurology, Università degli Studi di Milano, and Ospedale S. Raffaele, Via Olgettina 58, 20132 Milano, Italy

A Nicosia

Istituto di Ricerche di Biologia Molecolare P.Angeletti, Via Pontina Km. 30.6, 00040 Pomezia RM, Italy

R Cortese

Istituto di Ricerche di Biologia Molecolare P.Angeletti, Via Pontina Km. 30.6, 00040 Pomezia RM, Italy

Phage displayed random peptide libraries were screened in order to identify phagotopes reacting with the IgG present in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients. Six families of phagotopes each composed of different isolates were identified. These phagotopes were used as reagents, to characterise IgG present in the CSF of MS patients. The following results were obtained: (a) CSF antibodies from different patients display different specificities; (b) anti-phagotope antibodies are also present in the serum of MS patients; (c) Anti-phagotope antibodies are equally frequent in the serum of MS patients and of healthy individuals; (d) some of the anti-phagotope antibodies are enriched in the CSF of MS patients. These data show that the natural antigen(s) recognised by CSF antibodies is rather common in the general population. By using the selected phagotopes as immunogens in rabbits, we have derived large quantities of anti-phagotope antisera which can provide for useful tools toward the identification of the natural antigen(s) recognised by MS CSF antibodies.

Key Words: multiple sclerosis • oligoclonal bands • CSF • phage-libraries

Multiple Sclerosis, Vol. 4, No. 1, 31-36 (1998)
DOI: 10.1177/135245859800400108


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