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T cell receptor Vb5 and Vb17 clonal diversity in cerebrospinal fluid and peripheral blood lymphocytes of multiple sclerosis patients

Cellular Immunology Laboratory, Hopital de la Salpêtrière, 75651 Paris cedex 13, France, INSERM CJF 9608, Hopital de la Salpêtrière, 75651 Paris cedex 13, France

D Chabas

Cellular Immunology Laboratory, Hopital de la Salpêtrière, 75651 Paris cedex 13, France, INSERM CJF 9608, Hopital de la Salpêtrière, 75651 Paris cedex 13, France

B Duprey

Cellular Immunology Laboratory, Hopital de la Salpêtrière, 75651 Paris cedex 13, France

O Lyon-Caen

Fédération de Neurologie, Hopital de la Salpêtrière, 75651 Paris cedex 13, France

R Liblau

Cellular Immunology Laboratory, Hopital de la Salpêtrière, 75651 Paris cedex 13, France, INSERM CJF 9608, Hopital de la Salpêtrière, 75651 Paris cedex 13, France

To better characterize the cellular immune response taking place in the MS central nervous system, we investigated the blood and CSF T cell receptor (TCR) Vß5 and Vb17 repertoire in HLA-typed patients with recently diagnosed MS or other neurological diseases (OND). Using a RT-PCR based technique, we analysed directly ex vivo the CDR3 size of TCR ß chains utilizing Vß5 (eight patients with MS and one with OND) or Vß17 (eight patients with MS and six with OND) gene segments on paired blood-CSF samples. Globally, the analysis of Vß5-Jß and Vß17-Jß repertoire showed a less diverse pattern in the CSF samples than in the corresponding peripheral blood lymphocytes both in MS and in OND patients. However, we did not detect any recurrent clonal expansion within the Vb5⁺ T cells in MS patients, underlining the potential limits of Vß5- based immunotherapy in MS. We found an expanded T cell population using the same Vß17-Jß1.6 combination with identical CDR3 length in the CSF of three MS patients and none of the control patients. These results suggest selective expansion of T cells expressing this segment gene in the MS central nervous system.

Key Words: multiple sclerosis • cerebrospinal fluid • T cell • T cell receptor • autoimmunity • immunotherapy

Multiple Sclerosis, Vol. 4, No. 3, 154-161 (1998)
DOI: 10.1177/135245859800400313


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