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Cytokine analysis in multiple sclerosis by competitive RT - PCR: A decreased expression of IL-10 and an increased expression of TNF- in chronic progression

Department of Neurology, Karolinska Institute, Huddinge University Hospital, Huddinge, Sweden

Ping Huang

Department of Neurology, Karolinska Institute, Huddinge University Hospital, Huddinge, Sweden

Hans Link

Department of Neurology, Karolinska Institute, Huddinge University Hospital, Huddinge, Sweden

Jan Hillert

Department of Neurology, Karolinska Institute, Huddinge University Hospital, Huddinge, Sweden, Center for BioTechnology, NOVUM, Huddinge, Sweden

Multiple sclerosis (MS) is an inflammatory, demyelinating disease that is specific to the central nervous system. Cytokines are thought to be key mediators of the autoimmune attack against central nervous system myelin in MS. To investigate the involvement of cytokines in MS, the mRNA levels of interferon gamma (IFN-g), tumor necrosis factor alpha (TNF-a), interleukin-4 (IL-4) and interleukin-10 (IL-10) in peripheral blood mononuclear cells without stimulation in vitro were quantified by a competitive reverse transcription polymerase chain reaction technique. The level of IL-10 specific mRNA was significantly decreased in 47 MS patients compared with 42 healthy controls (P50.0001). TNF-a was significantly increased in MS patients compared with healthy controls (P=0.014), especially in the patients with chronic progressive MS (P=0.0003). Thus we conclude that there are significant in vivo alterations in cytokine gene expression in the periphery in MS.

Key Words: multiple sclerosis • tumor necrosis factor-alpha • interferon-gamma • interleukin-4 • interleukin-10 • reverse transcription polymerase chain reaction (RT-PCR)

Multiple Sclerosis, Vol. 5, No. 5, 342-348 (1999)
DOI: 10.1177/135245859900500507


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