This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Bartnik, B L Articles by Devon, R M Search for Related Content PubMed PubMed Citation Articles by Bartnik, B L Articles by Devon, R M Social Bookmarking                   What's this?

Macrophages: their myelinotrophic or neurotoxic actions depend upon tissue oxidative stress

Department of Anatomy and Cell Biology and The Cameco Multiple Sclerosis and Neuroscience Research Centre, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5 Canada

B HJ Juurlink

Department of Anatomy and Cell Biology and The Cameco Multiple Sclerosis and Neuroscience Research Centre, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5 Canada

R M Devon

Department of Oral Biology, and The Cameco Multiple Sclerosis and Neuroscience Research Centre, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5 Canada

There are still questions regarding whether macrophages found in MS lesions are agents of recovery or of destruction. To address this, we examined in aggregate cultures prepared from dissociated embryonic spinal cord tissue, with or without addition of exogenous macrophages, the effect of menadione-induced oxidative stress. Similar to findings of other laboratories, we observed that in the absence of oxidative stress macrophage enrichment promoted myelinogenesis. In macrophage-poor cultures, menadione at 5 µM had very little effect upon the status of the aggregate cultures; however, increasing this to 10 and 20 µM did result in some damage to axons and myelin. By contrast, in macrophage enriched cultures, menadione at a concentration as little as 5 µM caused the complete destruction of the aggregates. We suggest that in neural tissues that have sufficiently high macrophage numbers, oxidative stress results in a positive inflammatory feedback loop that results in massive tissue destruction. We further suggest that what we see in macrophage-enriched aggregates subjected to oxidative stress may represent what happens in the Marburg-type of MS lesion.

Key Words: cell culture • demyelinating disease • inflammation • myelin • multiple sclerosis • oligodendroglia

Multiple Sclerosis, Vol. 6, No. 1, 37-42 (2000)
DOI: 10.1177/135245850000600108


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K.M. Mitchell, A.L. Dotson, K.M. Cool, A. Chakrabarty, S.H. Benedict, and S.M. LeVine Deferiprone, an orally deliverable iron chelator, ameliorates experimental autoimmune encephalomyelitis Multiple Sclerosis, November 1, 2007; 13(9): 1118 - 1126. [Abstract] [PDF]