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Raised cerebrospinal fluid nitrite and nitrate levels in patients with multiple sclerosis: no correlation with disease activity

J Drulovi

Institute of Neurology, Clinical Centre of Serbia, Dr Subotia 6, 11 000 Belgrade, Yugoslavia

I Dujmovi

Institute of Neurology, Clinical Centre of Serbia, Dr Subotia 6, 11 000 Belgrade, Yugoslavia

Mesaro

Institute of Neurology, Clinical Centre of Serbia, Dr Subotia 6, 11 000 Belgrade, Yugoslavia

T Samardi

Institute for Biological Research, 29 novembra 142, Belgrade 11000, Yugoslavia

D Maksimovi

Institute for Biological Research, 29 novembra 142, Belgrade 11000, Yugoslavia

N Stojsavljevi

Institute of Neurology, Clinical Centre of Serbia, Dr Subotia 6, 11 000 Belgrade, Yugoslavia

Z Levi

Institute of Neurology, Clinical Centre of Serbia, Dr Subotia 6, 11 000 Belgrade, Yugoslavia

M Mostarica Stojkovi

Institute of Microbiology and Immunology, University of Belgrade, School of Medicine, Dr Subotia 1, Belgrade, Yugoslavia

A growing body of evidence implicates excessive generation of nitric oxide (NO) within the central nervous system (CNS) in multiple sclerosis (MS). The aim of our study is to analyse nitrite and nitrate as end products of NO in the cerebrospinal fluid (CSF) from MS patients and correlate the concentrations with clinical characteristics of the disease. CSF nitrite and nitrate concentrations were measured after reduction of nitrate, by Griess reaction, in 105 MS patients, 27 patients with non-inflammatory neurological disorders (NIND) and 13 individuals without neurological disorder (Co). Mean CSF nitrite and nitrate concentrations were significantly higher in patients with MS and NIND compared with the Co patients (9.44 and 8.68, respectively, versus 6.85 µM; P=0.0001 and P=0.031, respectively). There was no significant correlation between CSF nitrite and nitrate concentrations and activity, phase, severity and duration of MS. Our data are in agreement with the results of previous studies which have demonstrated raised concentrations of CSF NO metabolites in MS patients, providing further evidence for NO involvement in MS. The lack of correlation between NO metabolites and disease activity speaks in favour of the possible dual role of NO, as both immunoregulatory and proinflammatory molecule, in the pathogenesis of MS.

Key Words: multiple sclerosis • nitric oxide • cerebrospinal fluid • magnetic resonance imaging • disease activity • brain tumour

Multiple Sclerosis, Vol. 7, No. 1, 19-22 (2001)
DOI: 10.1177/135245850100700104


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